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. 2014 Jul 9;18(4):230. doi: 10.1186/cc13983

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Copyright © 2014 Iba et al.; licensee BioMed Central Ltd.

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Neutrophil adhesion to blood vessels. Left panel: intra-vital microscopic view of the mouse mesenteric vein at 1 hour after lipopolysaccharide infusion. The leukocyte (arrow) adheres to the endothelium and the platelets aggregate in the surrounding area (arrow heads) (objective lens × 20). Right panel: fluorescence live imaging of the mesenteric vein in mouse showing leukocytes in the thrombus (arrows). Fluorescent microscopic examination was performed at 1 hour after infusion of lipopolysaccharide and 4′,6-diamidino-2-phenylindole (DAPI). Nuclei of the damaged endothelial cells were also stained in blue (arrow head) (objective lens × 40).