Figure 5.

Interleukin 27–treated dendritic cells inhibited types 1 and 17 T helper cell response. (A) and (B) Allogeneic CD4+ T cells were cocultured with dendritic cells (DCs) or interleukin 27 (IL-27)–treated DCs (monocyte-derived DCs from four healthy controls and four patients with active BD) at a ratio of 5:1 for 4 days. Interferon γ (IFN-γ) and IL-17 production in the cell culture supernatants was determined by enzyme-linked immunosorbent assay. (C) Intracellular expression of IFN-γ and IL-17 was analyzed by flow cytometry. Representative flow cytometry dot plots for each group are shown. (D) and (E) Quantitative analysis of the frequency of IL-17-producting and IFN-γ-producing CD4+ T cells was carried out by paired-samples t-tests for related samples. All of the data are representative of three independent experiments and are expressed as average ± standard deviation.