Figure 4.

Effect of β-catenin knockdown or NFAT inhibitor on integrins, Shh/Gli, and Notch/Hes-1 gene expression. mRNA expression levels of integrin α2, integrin β1, Shh, Gli1, Notch, and Hes-1 were analyzed after cells were transfected with β-catenin-specific siRNA for 48 h or treated with NFAT inhibitor VIVIT (500 nM). A representative result from four independent experiments is shown. *P < 0.05 versus control (tissue culture polystyrene); ^# P < 0.05 versus pretreatment substrate; ^$ P < 0.05 versus modified PT substrate; ^@ P < 0.05 versus sand-blasted, large-grit acid-etched (SLA) substrate;**P < 0.05 versus modified SLA substrate.