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. 2014 Jun 25;2014:452175. doi: 10.1155/2014/452175

Table 1.

Primer sequences used for real-time RT-PCR analysis of gene expression.

Gene Forward primer (5′-3′) Reverse primer (5′-3′)
RUNX2 GTCTCACTGCCTCTCACTTG CACACATCTCCTCCCTTCTG
OSX TGAGGAGGAAGTTCACTATGG TTCTTTGTGCCTGCTTTGC
FOSB TCCAGGCGGAGACAGATCAGTTG TCTTCGTAG GGGATCTTGCAGCC
FRA1 CCCTGCCGCCCTGTACCTTGTATC AGACATTGGCTAGGGTGGCATCTGCA
Wnt3a GTCCCGTCCCTCCCTTTC ACCTCTCTTCCTACCTTTCCC
Wnt5a TCTCAGCCCAAGCAACAAGG GCCAGCATCACATCACAACAC
β-catenin GGCAGCAACAGTCTTACC TCCACATCCTCTTCCTCA
Integrin α2 ACTGTTCAAGGAGGAGAC GGTCAAAGGCTTGTTTAGG
Integrin β1 ATTACTCAGATCCAACCAC TCCTCCTCATTTCATTCATC
Calmodulin CAGATATTGATGGAGACGGA GAGCACACGAAGTACAAGAG
NFATc1 CCTTCGGAAGGGTGCCTTTT AGGCGTGGGGCCTCAGCAGG
Shh CGCCAGCGGAAGGTATGAAG CAACTTGTCCTTACACCTCTGAGTC
Gli1 AATGCTGCCATGGATGCTAGA GAGTATCAGTAGGTGGGAAGTCCATAT
Notch GCCGCCTTTGTGCTTCTGTTC CCGGTGGTCTGTCTGGTCGTC
Hes-1 AGGCGGACATTCTGGAAATG CGGTACTTCCCCAGCACACTT
GAPDH GCTCTCCAGAACATCATCC TGCTTCACCACCTTCTTG