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. 2014 Jun 27;20:82–92. doi: 10.12659/MSMBR.890858

Figure 2.

Figure 2

Inhibitory effect of resveratrol on HG-induced ROS overproduction and p47 phox expression in VSMCs. Cells were pretreated with resveratrol for 24 h prior to HG exposure for 30 min. ROS were detected using a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) probe. (A) Representative images of ROS fluorescence in different groups: NG (5 mM) group, HG (30 mM) group, and groups of VSMCs treated with different concentrations of resveratrol (1, 10, 50, and 100 mM) prior to HG exposure. (B) Fluorescence intensity measured by spectrofluorometer. (C) Blotting data for phospho-p47 phox and β-actin protein expressions from the VSMCs. Values are expressed as means ±S.D. from 3 independent experiments. Bar: 200 μm. # P<0.05 indicates significant difference from the HG (30 mM) group. * P<0.01 indicates significant difference from the HG (30 mM) group.