Fig. 1. Compartments isolated by LCM.
Some of the compartments isolated by LCM are illustrated. At E8.5 the cranial mesenchyme (MES), with neural crest and paraxial mesoderm, as well as the flanking neuroepithelium, divided into floorplate (FP) and more dorsal non-floor plate (NFP), as well as more caudal neuroepithelium (CN). At E9.5 LCM was used to purify the cranial mesenchyme (MES), the flanking neuroepithelium (NE), the epidermal ectoderm, including the olfactory placode (EE), as well as the otic vesicle, mandibular and maxillary arches (not shown). At E10.5 we collected the lateral nasal prominence (LNP), medial nasal prominence (MNP), flanking neuroepithelia (L-NE, M-NE, C-NE), olfactory pit (OP), and, Rathke’s pouch, both mandibular and maxillary arches, including both mesenchyme and epidermal ectoderm compartments (not shown).