Fig. 6.

An independent role for Perd function in myofibrillogenesis. (A–B′) Confocal micrographs of muscles stained with Rhodamine–Phalloidin (red) at the end of pupal development. Dotted lines and white brackets indicate muscle width and attachment surface, respectively. (A) Control dorsal abdominal muscles. (A′) Magnification of a control muscle showing its muscle attachment surface and its width, which can also be seen in the cross section in A′. (B) perd-depleted abdomens, where RNAi was induced starting at 30–40 hours APF, once the muscle migration has finished and the muscle attachments have been established. (B′) Magnification of a perd-depleted muscle. Note that this muscle is thinner even though it has an attachment surface similar to the control. A cross-section of this muscle shows that it contains less myofibrils than the control. (C) Scatter plot of muscle widths (y-axis) in relation to their attachment surface (x-axis) in control muscles, in muscles where perd-RNAi was expressed starting from larval (L) period 1 to 2, and in muscles where perd-RNAi was expressed starting from 30 to 40 h APF. A3, A4 and A5 indicate the corresponding abdominal segments. Scale bars: 5 µm.