| Template (25–200 ng/μl) | x μl |
| 10 x Buffer (with MgCl2) | 5.0 μl |
| 2.5 mM dNTP | 4.0 μl |
| 10 μM primer-Forward | 2.0 μl |
| 10 μM primer-Reverse | 2.0 μl |
| Taq (1.25 U/μl) | 0.25 μl |
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