Figure 3. The major component of acidosis-induced Ca²⁺ mobilization is via Ca²⁺ entry through ASICs in HT29 cells.

A: Superperfusion with Ca²⁺-containing solution (pH 5.0) induced a rise in [Ca²⁺]_cyt in a control experiment. The acidosis-induced rise in [Ca²⁺]_cyt was significantly reduced by amiloride (100 μM, B), removal of extracellular Ca²⁺ (C), or SK&F96365 (30 μM, D). E: Summarized data showing net peak of [Ca²⁺]_cyt rise induced by pH 5.0 in the cells exposed to different treatments. Values are mean ± SE, n =47–50 cells for each tracing or bar. **P < 0.01 vs. control with Ca²⁺-containing solution in the absence of inhibitors.