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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Food Chem Toxicol. 2014 May 16;70:114–119. doi: 10.1016/j.fct.2014.05.002

Fig. 3.

Fig. 3

EGCG induced fewer DNA double-strand breaks, as detected in the neutral comet assay, in the presence of α-amylase in HeLa (A and B) and p53R (C and D) cells. To confirm our findings from the p53R assay, the neutral comet assay was performed on HeLa or p53R cells, treated with two different doses of EGCG (10 μg/ml and 20 μg/ml: doses at which the peak activity was seen in individual p53R assays) for 30 min in the presence or absence of α-amylase at 50 units/ml. At least 70 cells were analyzed per sample to calculate the mean tail moment of each cell population. Representative images of the cells are shown in (A) and (C). The mean tail moment for each cell population and the standard error of the mean reflecting between-cell variation in the same experiment are shown in (B) and (D).