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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Arthritis Rheum. 2013 Jan;65(1):159–166. doi: 10.1002/art.37750

Figure 3.

Figure 3

Elevated levels of matrix metalloproteinase 8 (MMP-8), MMP-9, and MMP-13 protein in SIRT-1y/y (y/y) mouse cartilage. A, Immunohistochemical staining for MMP-8, MMP-9, and MMP-13 in the paws of 5 month-old wild-type (WT) and y/y mice. Hematoxylin counterstained; original magnification × 20. B, Percent of cells stained positively for MMP-8, MMP-9, and MMP-13 in the WT and y/y mice. The percent of positively stained cells per field was determined for an average of 6 fields in 3 sections from 3 separate mice per genotype. Bars show the mean ± SD. * = P = 5.0416 × 10−6 for MMP-8, P = 7.651 × 10−6 for MMP-9, and P = 1.7189 × 10−5 for MMP-13, versus y/y mice. C, Immunoblotting of protein extracts from WT and y/y mice for MMP-8, MMP-9, and MMP-13 protein levels. Results are representative of 3 different experiments and were consistent with the results of the immunohistochemical analysis.