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Pyrene-based fluorescence analysis of polyaspartamide polymersomes. (a) The fluorescence intensity ratio while the pyrene was incorporated into a suspension of MA-0.0, MA-2.7, MA-4.8, and PHEA in phosphate buffered saline (PBS), respectively. (b) The critical aggregation concentration (CAC), marked by the turning point of I3/I1 values in part a, of MA-0.0, MA-2.7, and MA-4.8 in PBS.
