Figure 3. Pou3f1 promotes neural fate commitment in vivo.
(A) Whole-mount in situ hybridization of Pou3f1 in early mouse embryos (E5.5–E8.0). The arrowhead marks the position-plane of the transverse section of the corresponding embryo below. Scale bars, 100 μm. (B) Contribution of injected GFP-labeled control (Ctrl), Pou3f1-knockdown (Pou3f1-KD), and inducible Pou3f1-overexpressing (Pou3f1-OE) ESCs to different germ lineages in chimeric embryos. NE, neuroectoderm; M, mesenchyme; and S, somite. Scale bars, 50 μm. (C) Statistical analysis of GFP-positive cell distribution in the various germ layer lineages in the ESC blastocyst injection study. The values represent the mean ± SD for C.
Figure 3—figure supplement 1. Information of chimeric mice generated from Pou3f1-overexpressing or knockdown ESCs.
