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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Ann N Y Acad Sci. 2014 Apr 15;1314:64–68. doi: 10.1111/nyas.12422

Figure 2.

Figure 2

ATP7A is a mobile and versatile copper-transporting ATPase. (A) Confocal images of HEK293T cells transfected with a Venus-tagged version of wild-type ATP7A relocalizes from the trans-Golgi network under basal copper cellular conditions (0.5 μM) and to the plasma membrane under increased copper (200 μM). (B) Total internal reflection fluorescence (TIRF) microscopy in normal human fibroblasts stained with an antibody against ATP7A illustrates the same phenomenon. TIRF selectively illuminates the plasma membranes of cells.