Table 17.3.2.
Interaction Trap Yeast Selection Strainsa
| Strain | Relevant genotype | Number of operators | Comments/description |
|---|---|---|---|
| EGY48b | MATα trp1, his3, ura3, lexAops-LEU2 | 6 | lexA operators direct transcription from the LEU2 gene; EGY48 is a basic strain used to select for interacting clones from a cDNA library |
| EGY191 | MATα trp1, his3, ura3, lexAops-LEU2 | 2 | EGY191 provides a more stringent selection than EGY48, producing lower background with baits with instrinsic ability to activate transcription |
| L40b | MATα trpl, leu2, ade2, GAL4, lexAops-HIS34, lexAops-lacZ8 | Expression driven from GAL1 promoter is constitutive in L40 (inducible in EGY strains); selection is for HIS prototrophy. Integrated lacZ reporter is considerably less sensitive than pSH18-34 maintained in EGY strains. |
a
Interaction Trap reagents represent the work of many contributors. The original basic reagents were developed in the Brent laboratory (Gyuris et al., 1993). Strains for specialized applications have been developed by the following individuals: E. Golemis, Fox Chase Cancer Center, Philadelphia, Pa. (EGY48, EGY191); A.B. Vojtek and S.M. Hollenberg, Fred Hutchinson Cancer Research Center, Seattle, Wash. (L40).
b
Strains commercially available from OriGene.