Table 17.3.3.
Source of RNA/DNA | Independent clones | Insert sizeb |
---|---|---|
Cell lines | ||
HeLa cells (human cervical carcinoma) | 9.6 × 106 | 0.3–3.5 kb (1.5 kb) |
WI-38 cells (human lung fibroblasts), serum-starved, cDNA | 5.7 × 106 | 0.3–3.5 kb (1.5 kb) |
Jurkat cells (human T cell leukemia) | 5.7 × 106 | (>1.3) |
SKOV3 human Y ovarian cancer | 5.0 × 106 | (>1.4 kb) |
MDBK cell, bovine kidney | 5.8 × 106 | (>1.2 kb) |
MCF7 breast cancer cells, untreated | 1.0 × 107 | (>1.5 kb) |
MCF7 breast cancer cells, estrogen-treated | 1.0 × 107 | (>1.1 kb) |
MCF7 breast cancer cells, serum-grown | 1.0 ×107 | 0.4–3.5 kb |
LNCAP prostate cell line, untreated | 2.9 × 106 | (>0.8 kb) |
LNCAP prostate cell line, androgen-treated | 4.6 × 106 | (>0.9 kb) |
Tissues | ||
Human liver | 1.1 × 107 | (>1 kb) |
Human ovary | 4.6 × 106 | (>1.3 kb) |
Human peripheral blood leukocyte | 1.0 × 107 | (>1.3 kb) |
Human fetal kidney | 3.0 × 106 | (>1 kb) |
Human prostate | 1.4 × 106 | (>1 kb) |
Human prostate cancer | 1.1 × 106 | (>0.9 kb) |
Human fetal liver | 8.6 × 106 | (>1 kb) |
Human fetal brain | 3.5 × 106 | 0.5–1.2 kb (1.5 kb) |
Mouse brain | 6.1 × 106 | (>1 kb) |
Mouse breast, lactating | 1.0 × 107 | 0.4–3.1 kb |
Mouse breast, involuting | 1.0 × 107 | 0.4–7.0 kb |
Mouse breast, virgin | 1.0 × 107 | 0.4–5.5 kb |
Mouse breast, 12 days pregnant | 6.3 × 106 | 0.4–5.3 kb |
Mouse skeletal muscle | 7.2 × 106 | 0.4–3.5 kb |
Rat adipocyte, 9-week-old Zucker rat | 1.0 × 107 | 0.4–5.0 kb |
Rat brain | 4.5 ×106 | 0.3–3.4 kb |
Rat testis | 8.0 × 106 | (>1.2 kb) |
Rat thymus | 8.2 × 106 | (>1.3 kb) |
Mouse liver | 9.5 × 106 | (>1.4 kb) |
Mouse spleen | 1.0 × 107 | (>1 kb) |
Mouse ovary | 4.0 × 106 | (>1.2 kb) |
Mouse prostate | 3.7 × 106 | 0.3–0.4 kb |
Mouse embryo, whole (19-day) | 1.0 × 105 | 0.2–2.5 kb |
D. melanogaster, adult, cDNA | 1.8 × 106 | (>1.0 kb) |
Miscellaneous | ||
S. cerevisiae, S288C, genomic | 4.0 × 106 | 0.5–4.0 kb |
C. elegans | 3.8 × 106 | (>1.2 kb) |
All libraries were constructed in conjunction with the laboratory of Roger Brent (brent@molsci.org) and are available from OriGene. All were constructed in the pJG4-5 vector, except the SKOV3 human Y ovarian cancer line, which was constructed in the pYESTrp vector.
Average insert size given in parentheses.