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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Curr Protoc Cell Biol. 2001 May;0 17:Unit–17.3. doi: 10.1002/0471143030.cb1703s08

Table 17.3.3.

Libraries Compatible with the Interaction Trap System

Source of RNA/DNA Independent clones Insert sizeb
Cell lines
HeLa cells (human cervical carcinoma) 9.6 × 106 0.3–3.5 kb (1.5 kb)
WI-38 cells (human lung fibroblasts), serum-starved, cDNA 5.7 × 106 0.3–3.5 kb (1.5 kb)
Jurkat cells (human T cell leukemia) 5.7 × 106 (>1.3)
SKOV3 human Y ovarian cancer 5.0 × 106 (>1.4 kb)
MDBK cell, bovine kidney 5.8 × 106 (>1.2 kb)
MCF7 breast cancer cells, untreated 1.0 × 107 (>1.5 kb)
MCF7 breast cancer cells, estrogen-treated 1.0 × 107 (>1.1 kb)
MCF7 breast cancer cells, serum-grown 1.0 ×107 0.4–3.5 kb
LNCAP prostate cell line, untreated 2.9 × 106 (>0.8 kb)
LNCAP prostate cell line, androgen-treated 4.6 × 106 (>0.9 kb)
Tissues
Human liver 1.1 × 107 (>1 kb)
Human ovary 4.6 × 106 (>1.3 kb)
Human peripheral blood leukocyte 1.0 × 107 (>1.3 kb)
Human fetal kidney 3.0 × 106 (>1 kb)
Human prostate 1.4 × 106 (>1 kb)
Human prostate cancer 1.1 × 106 (>0.9 kb)
Human fetal liver 8.6 × 106 (>1 kb)
Human fetal brain 3.5 × 106 0.5–1.2 kb (1.5 kb)
Mouse brain 6.1 × 106 (>1 kb)
Mouse breast, lactating 1.0 × 107 0.4–3.1 kb
Mouse breast, involuting 1.0 × 107 0.4–7.0 kb
Mouse breast, virgin 1.0 × 107 0.4–5.5 kb
Mouse breast, 12 days pregnant 6.3 × 106 0.4–5.3 kb
Mouse skeletal muscle 7.2 × 106 0.4–3.5 kb
Rat adipocyte, 9-week-old Zucker rat 1.0 × 107 0.4–5.0 kb
Rat brain 4.5 ×106 0.3–3.4 kb
Rat testis 8.0 × 106 (>1.2 kb)
Rat thymus 8.2 × 106 (>1.3 kb)
Mouse liver 9.5 × 106 (>1.4 kb)
Mouse spleen 1.0 × 107 (>1 kb)
Mouse ovary 4.0 × 106 (>1.2 kb)
Mouse prostate 3.7 × 106 0.3–0.4 kb
Mouse embryo, whole (19-day) 1.0 × 105 0.2–2.5 kb
D. melanogaster, adult, cDNA 1.8 × 106 (>1.0 kb)
Miscellaneous
S. cerevisiae, S288C, genomic 4.0 × 106 0.5–4.0 kb
C. elegans 3.8 × 106 (>1.2 kb)
a

All libraries were constructed in conjunction with the laboratory of Roger Brent (brent@molsci.org) and are available from OriGene. All were constructed in the pJG4-5 vector, except the SKOV3 human Y ovarian cancer line, which was constructed in the pYESTrp vector.

b

Average insert size given in parentheses.

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