. Author manuscript; available in PMC: 2014 Jul 14.

Published in final edited form as: Curr Protoc Cell Biol. 2001 May;0 17:Unit–17.3. doi: 10.1002/0471143030.cb1703s08

Table 17.3.4.

Two-Hybrid System Variants

System^a	DNA-binding domain	Activation domain	Selection	Originating laboratory^b
Two-hybrid	GAL4	GAL4	lacZ, HIS3	S. Fields
Improved two-hybrid	GAL4	GAL4	HIS3, lacZ	S. Elledge
ProQuest	GAL4	GAL4	HIS3, URA3, lacZ	Invitrogen
Matchmaker System 3	GAL4	GAL4	ADE2, HIS3, lacZ, MEL1	Clontech
Interaction trap	LexA	B42	LEU2, lacZ	R. Brent
Modified two-hybrid	LexA	VP16	HIS3, lacZ	S. Hollenberg
Grow‘n’Glow	LexA	B42	LEU2, GFP	S. Cormack
Dual bait	LexA/cI	B42	LEU2, lacZ / LYS2, GusA	E. Golemis
Two-bait interaction trap	LexA/TetR	B42	lacZ/URA3	R. Brent
Grow ‘n’ Glow ACE1	Ace1	Ace1	CUP1, GFP	T. Munder

Only key systems designed to detect protein-protein interactions are listed. A variety of specialized two-hybrid systems, including systems designed to work with self-activating and membrane proteins, are described in a recent review (Izumchenko et al., 2007).

For references see Izumchenko et al. (2007).