Figure 3. A substantial fraction of isolates in a large panel are neutralized by high-mannose patch bnMAbs when the N332 or N334 glycan sites are eliminated by alanine mutagenesis.

Antibody families were tested on an 80-virus panel, whose members naturally include a glycan site at N332/334 (A) and on the same panel with the glycan site at N332/334 removed by alanine mutagenesis (B). Listed are percent viruses neutralized at the indicated neutralization IC₅₀ cutoffs. Cumulative distribution frequency (CFD) of percent viruses neutralized in the 80-virus panel with and without the N332/334 glycan site present at different IC₅₀ cutoffs for the (C) PGT121 antibody family and the (D) PGT128 antibody family.