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. Author manuscript; available in PMC: 2015 Jul 20.
Published in final edited form as: J Biotechnol. 2014 Apr 24;0:19–29. doi: 10.1016/j.jbiotec.2014.04.011

Table 1.

Glycoenzyme constructs used in this study.

Glycoenzyme* ie1 plasmid (reference) 39K plasmid Method used for 39K plasmid construction
MGAT1 pIE1GlcNAcTI (Hollister et al., 2002) p39KHRGlcNAcTI 2.7 kb NotI-HindIII fragment from pIE1GlcNAcTI subcloned into NotI-HindII sites of p39K-SEAP
MGAT2 pIE1GlcNAcTII (Hollister et al., 2002) p39KHRGlcNAcTII 2.6 kb BglII-HindIII fragment from pIE1GlcNAcTII subcloned into BglII-HindIII sites of p39K- SEAP
B4GALT1 pIE1HR3HyGalT(F282) (Geisler, in prep) p39KHR3HyGalT(F282) 1.0 kb BglII-NruI fragment from pIE1HR3HyGalT(F282) subcloned into BglII-PmeI sites of p39K- SEAP
GNPE pIE1HREcGNPE (Geisler and Jarvis, 2012a) p39KHREcGNPE 2.0 kb BglII-HindIII fragment from pIE1EcGNPE subcloned into BglII-HindIII sites of p39K-SEAP
SAS pIE1HRMmSAS (Geisler and Jarvis, 2012a) p39KHRMmSAS 2.4 kb NotI-HindIII fragment from pIE1HRMmSAS subcloned into NotI-HindIII sites of p39K-SEAP
CMAS pIE1HRMmCSAS (Geisler and Jarvis, 2012a) p39KHRMmCSAS 2.1 kb BamHI-XbaI fragment from pIE1MmCSAS subcloned into BglII-XbaI sites of p39K-SEAP
CSAT pIE1-hCSAT (Mabashi-Asazuma et al., 2013) p39K-hCSAT 2.3 kb BglII-HindIII fragment from pIE1-hCSAT subcloned into BglII-HindII sites of p39K-SEAP
ST3GAL4 pIE1-hST3GAL4b (Mabashi, in prep) p39K-hST3GAL4b 1.8 kb BamHI-XbaI fragment from pIE1-hST3GAL4b subcloned into BglII-XbaI sites of p39K-SEAP
ST6GAL1 pIE1HRHyST6Δcys (Geisler, in prep) p39KHRHyST6Δcys 1.9 kb SacII(3′ overhangs removed with T4 DNA Polymerase)-XbaI fragment from pIE1HRHyST6Δcys subcloned into NotI(5′ overhangs filled with T4 DNA Polymerase)- XbaI sites of p39K-SEAP
*

HUGO nomenclature, except CSAT, whose HUGO symbol is SLC35A1