Table 2.
Antibodies used for immunohistochemistry and immunofluorescence.
| Antigen | Antibody | Dilution | Retrievement |
|---|---|---|---|
| IL8Rβ | SC-23811 | 1 : 50 | PH6 |
| RBL2 | SC-53641 | 1 : 50 | PH9 |
| RB1 | BD #5544136 | 1 : 20 | PH9 |
| TP53 | Calbio.OP43A | 1 : 100 | |
| CCNA | NM MS1061-S1 | 1 : 15 | |
| P21 | Millipore OP64 | 1 : 25 | |
| P15 | SC-56327 | 1 : 20 | |
| HP1γ | Millipore 05-690 | 1 : 100 | PH6 |
| KI67 | Dako IR-626 | 1 : 1 | PH6 |
SC: SantaCruz Biotechnology; BD: Becton Dickinson and Company; Calbio: calbiochem; NM: neomarker; epitope retrievement was performed at indicated PH by boiling for 5 minutes.