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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Am J Respir Cell Mol Biol. 2004 Apr 1;31(2):162–170. doi: 10.1165/rcmb.2003-0307OC

Figure 3.

Figure 3

Effect of polymyxin B on LPS or E. coli-induced decrease in mCD14 expression. Cells were pretreated with PBS or polymyxin B [10 ng/ml] for 30 min prior to the addition of PBS, LPS [100 ng/ml], or E. coli (1×106 CFU/ml) for 24 hours. The expression of mCD14 was measured by flow cytometry. (A) Representative histogram of mCD14 expression on rabbit AM pretreated with PBS or polymyxin B and then exposed to PBS or LPS for 24 hours. (B) Representative histogram of mCD14 expression on rabbit AM pretreated with PBS or polymyxin B and then exposed to PBS or E. coli for 24 hours. (C) Data from six separate experiments was normalized as a percentage of the mean fluorescent intensity (MFI) of the PBS control. The black bars are the data from AM pretreated with PBS and the gray bars are the data from AM pretreated with polymyxin B. Values are mean ± SEM. #: p= 0.003 for polymyxin B + 24 hours LPS group vs PBS + 24 hours (n=4). *: p= 0.02 for polymyxin B + 24 hour E. coli vs PBS + 24 hours E. coli (n=6).