Fig. 4.
The log transformed gene expression levels among all inbred Biomphalaria glabrata lines, calculated for each gene independently, were divided into three ranges. For each gene, low, medium and high groups are illustrated. Inbred lines are listed from most resistant (i170) to most susceptible (i163), and the spread cell counts (Cell #) and % susceptibility (%S) are shown for each. (A) Heat map for all genes and all inbred lines. (B) Heat map of NADPH oxidase components and peroxiredoxin 1 (prx1) from resistant (R) and susceptible (S) lines with similar spread cell counts. Expression of the gene for the inferred phox subunit (infPhox) is significantly higher in R than S lines. Two other components of the NADPH oxidase complex which were measured, nox2 and phox22, were not significantly higher in R lines but show trends towards higher expression in the R lines. Genes are abbreviated as follows, where bold numbers refer to Contig numbers in a preliminary draft of the snail genome (https://www.vectorbase.org/organisms/biomphalaria-glabrata): aif, allograft inflammatory factor; arg, arginase; c42, catalase 42; d584, dual oxidase 584; d303, dual oxidase 303; frep3, fibrinogen-related protein 3; g2402, glutathione peroxidase 2402; g97, glutathione peroxidase 97; g65, glutathione peroxidase 65; mif, macrophage migration inhibitory factor; nox2, NADPH oxidase 2; phox22, phagocyte oxidase 22; infPhox, inferred phox subunit; prx6, peroxiredoxin 6; prx4, peroxiredoxin 4; prx621, peroxiredoxin 621; prx1, peroxiredoxin 1; tal-1, T-cell acute lymphocytic leukaemia-1.