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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 18;1843(9):1818–1833. doi: 10.1016/j.bbamcr.2014.04.015

Figure 5.

Figure 5

Comparison of transactivation potential of MTF-1ΔTAD versus dnMTF-1. A) Nuclear localization of MTF-1ΔTAD and dnMTF-1 in control and Zn-treated cells. dnMTF-1 is constitutively localized to the nucleus independent of metals treatment. B) In transient transfection assays in Hepa1c1c7 cells transfected with 20 ng of zfMT-Luc, the dnMTF-1 was able to significantly inhibit endogenous MTF-1 signaling in response to Zn treatment. C) In transient transfection assays in MEF MTF-1 null cells, the dnMTF-1 was much more efficient at inhibiting MTF-1 activation of the zfMT-Luc construct compared to MTF-1ΔTAD. D) In transient transfection assays in MEF MTF-1 null cells transfected with 20 ng of zfMT-Luc, the dnMTF-1 was able to significantly inhibit both Zn and Cd-induced activation of the zfMT-Luc construct by either MTF-1 and MTF-1ΔCys. Error bars represent one standard deviation: n = 6 replicates. The results shown in each panel are representative of two independent experiments.