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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 18;1843(9):1818–1833. doi: 10.1016/j.bbamcr.2014.04.015

Figure 7.

Figure 7

Confirmation of microarray results with quantitative RT-PCR. Four genes that were significantly altered by inhibition of endogenous MTF-1 signaling in zebrafish embryos were selected for confirmation by qRT-PCR. Results are expressed as fold change from the 28 hpf non-injected control embryos, normalized to 18S ribosomal RNA levels. Different letters denote significant changes in response to microinjection of dnMTF-1 compared to eGPF mRNA (One-way ANOVA, p<0.05). Error bars represent one standard deviation: n = 3 replicates. The results shown in each panel are representative of two independent experiments.