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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 18;1843(9):1818–1833. doi: 10.1016/j.bbamcr.2014.04.015

Figure 8.

Figure 8

The role of MTF-1 in regulating zebrafish and human αA crystallin gene expression. A) A schematic of the promoter region of the αA crystallin gene from both human and zebrafish displaying the location of putative MRE motifs and highlighting the regions of the promoters cloned into the pGL4.10[luc2] vector (Promega Corporation, Madison, WI). B) In transient transfection assays, MEF MTF-1 null cells were transfected with 40 ng of zfαACry-Luc, 20 ng of zebrafish MTF-1 and 60 ng of dnMTF-1. The zfαACry-Luc construct was responsive to both Zn and Cd treatment in an MTF-1 independent manner. Overexpression of MTF-1 or MTF-1 and dnMTF-1 inhibited the metal-responsiveness. C) In transient transfection assays, Hepa1c1c7 cells were transfected with 40 ng of hαACry-Luc, 20 ng of mouse MTF-1 and 60 ng of dnMTF-1. The hαACry-Luc construct was responsive to both Zn and Cd treatment in an MTF-1 dependent manner. dnMTF-1 significantly inhibited the MTF-1 mediated activation of hfαACry-Luc. Error bars represent one standard deviation: n = 6 replicates. The results shown in each panel are representative of two independent experiments.