Figure 6.

Recovery of bone marrow niche after myelosuppression triggers the increase of ECM component synthesis by Mks in vivo. A) Schematic representation of the strategy adopted for Mk sorting in platelets depleted mice and 5-FU treated mice. Mk in mice injected with 4μg of anti GPIbα were sorted between at day 2.5 of treatment and just before the recovery of blood peripheral platelet count. In 5-FU treated mice, Mk were sorted at day 10 of treatment in juxtaposition of bone marrow and pheripheral blood count recovery. Right panel shows a representative hematoxylin & eosin staining of sorted Mks. Scale bar = 10 μm. B) RT-PCR of laminin, type IV collagen chains and fibronectin in Mks treated with PBS (Saline) or anti GPIbα. *p value < 0.05. C) RT-PCR of laminin, type IV collagen chains and fibronectin in Mks treated with PBS (Saline) or 5-FU. *p value 0.05, **p value 0.01. D) Western blotting analysis of ECM components level in Mks sorted from bone marrow cells after 60 hours of anti GPIbα antibody injection and PBS as control. E) Western blotting analysis of ECM components level in Mks sorted from bone marrow cells of mice myelosuppressed with 5-fluorouracil or PBS as control. β-Actin was revealed to demonstrate equal protein loading. The images are representative of three independent experiments.