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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Mol Cancer Res. 2014 Feb 26;12(5):784–794. doi: 10.1158/1541-7786.MCR-13-0638

Figure 5.

Figure 5

Overexpression of DDB2 in ovarian CSCs alters the properties of CSCs. CP70-ALDH+ (A and D), SKOV3 spheroids (B and E), and 2008C13-CD44+CD117+ (C and F) cells were transfected with DDB2-expressing vector for 2 days. The expression of DDB2 and CSC marker Nanog were determined using immunoblotting (A to C). The sphere formation was assessed in nonadherent conditions (D to F). Bar, SD; n = 3; **, P < 0.01. G and H, NOD/SCID mice were subcutaneously injected with 1 × 104 SKOV3 spheroids transiently transfected with either empty vectors or DDB2-expressing vectors. Representative image for tumor growth is shown (G). Tumor sizes were measured and the tumor growth was plotted (H); n = 5; bar, SD; *, P < 0.05 compared with the vector group at the same time point. I, the expression of IκBα was determined in various ovarian cancer cell lines with either stable overexpression or knockdown of DDB2. J, 2008C13-CD44+CD117+ cells and SKOV3 spheroids were transiently transfected with DDB2-expressing plasmids for 2 days and the expression of IκBα was detected using immunoblotting.