Table 1.
The signal size (ΔF/F: fractional change in fluorescence intensity) and signal-to-noise ratio (S/N) were evaluated for the largest signal obtained in each preparation. The signal size (ΔI/I: fractional change in transmitted light intensity) and S/N with NK2761 were also compared. The recordings were made with a magnification of ×4×1.67. n.d.: not detectable (S/N < 1.0).
Signal size and signal-to-noise ratio
| Dye | Preparation reference | Objective ×4
|
|
|---|---|---|---|
| Maximum F/F (×10−2) | S/N | ||
| RH414 | NP12 | 0.17 | 1.1 |
| NP13 | 0.18 | 1.1 | |
| NP17 | n. d. | ||
| RH795 | NP14 | 0.40 | 1.4 |
| NP20 | 0.42 | 1.2 | |
| di-4-ANEPPS | NP15 | 1.6 | 1.8 |
| NP16 | 1.5 | 2.1 | |
| NP25 | 1.7 | 2.5 | |
| NP59 | 1.1 | 2.8 | |
| di-2-ANEPEQ | NP23 | 1.4 | 4.3 |
| NP57 | 1.7 | 4.4 | |
| NP60 | 1.3 | 4.0 | |
| di-3-ANEPPDHQ | NP30 | 0.70 | 2.7 |
| NP58 | 0.81 | 3.0 | |
| NP61 | 0.75 | 2.7 | |
| di-4-AN(F)EPPTEA | NP50 | 1.5 | 1.5 |
| NP51 | 1.2 | 1.5 | |
| NP71 | 1.1 | 1.5 | |
| NP72 | 1.2 | 2.5 | |
| NP77 | 1.3 | 1.5 | |
| di-2-AN(F)EPPTEA | NP67 | 1.1 | 1.4 |
| NP68 | 1.1 | 1.5 | |
| NP73 | 1.5 | 1.5 | |
| NP75 | 1.5 | 1.8 | |
| di-2-ANEPPTEA | NP63 | 1.2 | 1.2 |
| NP66 | 1.1 | 1.1 | |
| NP69 | 1.4 | 1.1 | |
| NP74 | 1.5 | 1.8 | |
| NP84 | 1.0 | 1.3 | |
|
| |||
| NK2761 | NP11 | 0.35 | 24 |
| NP32 | 0.33 | 22 | |
| NP38 | 0.34 | 23 | |
| NP40 | 0.36 | 26 | |