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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Neurosci Res. 2013 Sep 16;91(12):1639–1650. doi: 10.1002/jnr.23287

Figure 1. DM-affected spinal cords display SOD1-positive aggregates in motor neurons and marked myelinated axon loss in white matter.

Figure 1

Immunohistochemical demonstration of SOD1-containing aggregates in motor neurons in the thoracic spinal cord (A,B). Numerous aggregates stained with an antibody directed against SOD1 were present in ventral horn motor neurons from a DM-affected PWC (B). Similar aggregates were present in all affected dogs of both PCW and Boxer breeds. These inclusions were not observed in thoracic cord motor neurons from unaffected dogs (representative preparation from a control PWC shown in B). Luxol fast blue and periodic acid-Schiff stained thoracic spinal cord from unaffected (C) and grade 4 (D) PWCs. Pallor of the peripheral white matter in (D) corresponds to axonal loss and demyelination (arrows). Higher magnification of thoracic white matter from unaffected (E) and grade 4 (F) PWCs stained with toluidine-blue. Substantial axonal loss with myelin pathology is seen in the PWC with advanced DM. Bars in (A), (C) and (E) indicate magnifications in (B), (D) and (F) respectively.