Fig. 5.

Treatment of mouse sciatic nerve MPNST in immunocompetent mice. Mouse M2 cells (5 × 10⁴) were implanted into the sciatic nerve of C57BL6/129SVSVImJ hybrid mice. (A) View of tumor in sciatic nerve 7 days postimplant (black arrow). (B) Tumors were injected with G47Δ (3 × 10⁶ pfu) on day 10, and mice were sacrificed 48 h later. X-Gal staining of G47Δ-infected cells (blue) in sagittal sections from same tumor. Upper, 4× obj; lower 10× obj. Nerve bundle (white arrow). (C) G47Δ (3 × 10⁶ pfu; n = 16) or PBS (n = 4) in 2 µL was injected on day 10 (dashed line). External tumor size was measured by caliper. *P < .05, unpaired t-test. (D) Neurologic score; P < .01, days 30–40; 2-way ANOVA (Bonferroni posttests). (E) Kaplan–Meier survival curve. P < .0001, log-rank test.