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Investigation of pH-responsive properties of representative PEG–polypeptide copolymers. (a) pH titration curve of copolymers 1 and 2. (b) Reduction of relative FRET efficiency upon lowering the pH from 7.4 to 4.5. (c) Principle of the FRET experiment carried out by forming a vesicle constituted from the respective copolymer tagged with the Cy5.5 and Cy7 FRET pair.
