Figure 6.
(A) Copolymer 1 vesicle stability on the basis of FRET association of the carrier components following systemic administration in non-tumor-bearing BALB/c mice. Top panel corresponds to λex = 640 nm and λem = 700 nm (donor channel), and bottom panel corresponds to λex = 640 nm and λem = 800 nm (FRET channel). (B) Biodistribution quantitation in necropsied tissue (Li, liver; S, spleen; K, kidneys; H, heart; and Lu, lungs) 24 h following systemic administration in BALB/c mice in both the donor and FRET channels. Fluorescence recovery from harvested tissue includes the background subtraction of tissue from an untreated control for removal of autofluorescence. The resultant quantification is normalized to the injected dose, based on the fluorescence intensity of the injected dose following IVIS imaging of the vial pre-injection.