Fig. 1.
Panc-02 cells express only CCK-A receptor mRNA and protein. a mRNA for only the CCK-A receptor is detected in Panc-02 cells not the CCK-B receptor by quantitative RT-PCR. Receptor mRNA levels are standardized to murine HPRT mRNA levels as an internal control. N.D. not detected. Columns represent the fold change in mRNA levels calculated from the mean relative quantity and bars represent a 95 % CI (RQ = 2−(ΔΔCT±CI)). b CCK-A receptor protein is present in Panc-02 cells by immunofluorescence. c No immunoreactivity is detected using a CCK-B receptor antibody indicating that Panc-02 cells express the CCK-A receptor and not the CCK-B receptor