Fig. 2.

Effects of diet and a CCK-A receptor antagonist on body weight, serum CCK levels, and serum glucose levels. a Mean weight gain of mice fed a low-fat diet, a high-fat diet, or normal diet. Animals on the high-fat diet (solid inverted triangles) gained significantly more weight than animals on a normal diet (solid circles) or low-fat diet (open circles), ***p = 2.67 × 10⁻⁶. No differences in food or water intake were noted among treatment groups. A slight decrease in body weight was observed in all the diet groups on Day 54 due to surgery to implant pancreatic cancers cells (n = 10–12 mice per diet group). b Serum CCK levels were increased tenfold in animals on the high-fat diet (gray bars, **p <0.01), whereas CCK serum levels were tenfold decreased in animals on the low-fat diet (white bars, *p < 0.05) compared to animals on a normal diet (black bar). Treatment with the CCK-A receptor antagonist had no effect on serum CCK levels independent of diet (bars with crosshatches, n = 5–6 animals per treatment group). c Serum glucose levels in mice fed a normal diet (black bar), high-fat diet with vehicle treatment (gray bar), or high-fat diet with CCK receptor antagonist treatment (gray bar with cross-hatches). Although a high-fat diet caused an increase in serum glucose (**p = 0.010), CCK receptor antagonist treatment had no significant effect on glucose levels