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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Nov 7;34(1):26–33. doi: 10.1161/ATVBAHA.113.302355

Figure 4.

Figure 4

Adenoviral vectors encoding G5G8 (AdG5G8) reduces lipogenic gene expression and lowers plasma triglycerides (TGs). Obese (db/db) male mice and their lean littermates were administered control or AdG5G8 virus. A, Total RNA was extracted from liver 72 hours after viral administration and was analyzed by reverse-transcriptase polymerase chain reaction for expression of lipogenic genes. B, Full-length and processed sterol regulatory element–binding protein (SREBP) 1 were analyzed by immunob-lotting in whole cell lysates and nuclear extracts, respectively. C, Densitometry of SREBP immunoblots. Signal intensities are normalized to calnexin (CNX) and expressed as percent of the control mean. D and E, Plasma and hepatic TGs were determined by enzymatic colorimetric assay. F, TG secretion rates were determined after injection of Triton WR 1339. Data are mean±SEM (n=3–4). *P<0.05, **P<0.01, ****P<0.0001.