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Hepatic fibrosis. Double immunolabelling for MSA (red) and TGF-β1 (brown), hematoxylin counterstain. BPDEC are increased in number, hypertrophied and strongly positive for TGF- β1. HSCs (arrow head) are immunoreactive for MSA. Some HSCs are immunoreactive for MSA and TGF- β1 (arrow). Bar = 20 μm 100X.
