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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Stem Cells. 2014 Jan;32(1):269–278. doi: 10.1002/stem.1545

Figure 1.

Figure 1

Characterization of induced pluripotent stem cells (iPSCs) with various JAK2-V617F compositions derived from a single polycythemia vera (PV) patient. Representative images of undifferentiated phenotypes and in vivo pluripotency assays of JAK2 wild-type (PVB1.11, shown in (A)) and JAK2 V617F (PVB1.4, shown in (B)) PV-specific iPSCs. Colonies cultured on mouse embryonic fibroblasts were stained with antibodies against pluripotency-related cell surface markers TRA-1–60 and SSEA4. The fixed colonies were also stained with antibodies against human embryonic stem cell-specific transcription factors NANOG and OCT-4. Both types of cells were injected into immunedeficient NOD/SCID IL2 receptor gamma knockout (NSG) mice for teratomas formation. H&E staining of the teratomas showed the tissue types of all three embryonic germ layers endoderm, mesoderm, and ectoderm, demonstrating the pluripotency of iPSCs. (C): Karyotyping of both types of iPSCs showed normal karyotype. Scale bar = 200 μm.