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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Stem Cells. 2014 Jan;32(1):269–278. doi: 10.1002/stem.1545

Figure 4.

Figure 4

JAK inhibitors inhibit erythroid differentiation of hematopoietic progenitors generated from diversified induced pluripotent stem cell (iPSC) lines. (A): CD34+ cells generated from iPSCs were cultured in erythroid differentiation conditions for 7 days in the absence or presence of JAK inhibitors. The fold expansion was calculated after counting total cell numbers at the end of the 7-day culture. (B): Erythroid differentiation was measured by flow cytometry for CD235a expression. Percentages of CD235a+ cells at the end of 7-day culture in each culture condition were displayed as “Percent of red cells” on the y-axis. V617F/V617F: the data are an average of results from PVB1.4 and PVB1.18 (n = 2). WT/V617F: the data are an average of two independent experiments from iPV183 (n = 2). WT/WT: the data are an average of results from BC1, TNC1, and PVB1.15 in INCB018424 experiments (n = 3); BC1 and PVB1.11 in TG101348 and CYT387 experiments (n = 2). Data are mean ± SD. Abbreviation: wt, wild type.