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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Apoptosis. 2012 Apr;17(4):349–363. doi: 10.1007/s10495-011-0673-2

Fig. 5.

Fig. 5

ER stress up-regulates TRAIL-R2 through a PERK-dependent, CHOP and Ire1-independent mechanism. MDA-MB231 cells were transfected with scrambled (c) or CHOP (a), IRE1α (b) or PERK (c, d) siRNAs for 48 h to silence protein expression. Transfected cells were then treated with tunicamycin for 15 h prior to the analysis of protein knockdown and TRAIL-R2 expression by western blotting (a, b, c) or cell surface levels of TRAIL-R2 by flow cytometry (d). Results are representative of at least three independent experiments