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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Apoptosis. 2012 Apr;17(4):349–363. doi: 10.1007/s10495-011-0673-2

Fig. 7.

Fig. 7

GRP78 knockdown up-regulates TRAIL-R2 expression and sensitizes cells to TRAIL-induced apoptosis through a PERK-dependent mechanism. a Cell lysates from MDA-MB231 cells transfected with siRNAs (50 nM) for 48 h were analyzed for protein expression by western blotting (upper panel) or cell surface expression by flow cytometry (lower panel) as described in “Materials and methods” section. b MDA-MB231 were transfected with siRNAs for 48 h and treated with TRAIL (500 ng/ml) for 24 h. Apoptosis was assessed as the percentage of cells with sub-G1 DNA content. Error bars represent S.D. from three independent experiments. * Indicate a statistically significant difference in sensitivity to TRAIL when comparing GRP78 siRNA-transfected cells with cells transfected with GRP78/PERK siRNAs. (P <0.05)