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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: J Am Chem Soc. 2013 Dec 27;136(1):299–310. doi: 10.1021/ja409801p

Figure 4.

Figure 4

Influence of ML or 1 on the formation of metal-free and metal-induced Aβ40/42 aggregates. (a) Scheme of the inhibition experiment. (b) Aβ species were visualized by gel electrophoresis using immunoblotting with an anti-Aβ antibody (6E10). Experimental conditions: Aβ (25 µM); CuCl2 or ZnCl2 (25 µM); ML or 1 (50 µM); 4, 8, or 24 h; pH 6.6 (for metal-free and Cu(ii) experiments) or 7.4 (for metal-free and Zn(ii) experiments); 37 °C; constant agitation. Lanes: (1) Aβ; (2) Aβ + 1; (3) Aβ + ML; (4) Aβ + [CuCl2 or ZnCl2]; (5) Aβ + [CuCl2 or ZnCl2] + 1; (6) Aβ + [CuCl2 or ZnCl2] + ML. (c) TEM images of the 24 h incubated samples from (b).