FIGURE 2.
Frequency of mononucleated cells positive for MyoD and/or myogenin and of cells fused into myotubes in C2 cultures initiated at ‘higher density’ and reacted via double immunofluorescene with antibodies against MyoD and myogenin. Frequencies are based on the data summarized in Table I. Note that except for the graphs summarizing the frequency of nuclei in myotubes and the frequency of cells with a myogenic phenotype (panels D and H) all other graphs demonstrating frequency of cells represent mononucleated cells only.
