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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Growth Factors. 1997;15(1):1–27. doi: 10.3109/08977199709002109

FIGURE 4.

FIGURE 4

Micrographs demonstrating C2 cultures which were immunoreacted with various combinations of mouse monoclonal and rabbit polyclonal antibodies and counter stained with DAPI. Panel A — reactivity with a monoclonal antibody against myogenin and a polyclonal antibody against MEF2A. Panel B — reactivity with a monoclonal antibody against myosin and a polyclonal antibody against MEF2A. Panel C — reactivity with a monoclonal antibody against MyoD and a polyclonal antibody against cyclin A. Panel D — reactivity with a monoclonal antibody against desmin and a polyclonal antibody against MyoD. Panel E — reactivity with a polyclonal antibody against Myf5. Reactivity with the monoclonal antibodies was visualized with a fluorescein-labeled secondary antibody and reactivity with the polyclonal antibodies was visualized with a rhodamine-labeled secondary antibody. Cultures were initiated in proliferation medium and switched following 24 hours into medium consisting of 2% PCS in MEM (±PDGF). The different panels represent cultures fixed following 1 to 4 days in culture. Arrows in parallel DAPI and immunofluorescent micrographs in panels A through D mark the position of cells positive for both antibodies examined while arrowheads in these panels mark the position of cells which are positive for only one of the two antibodies examined. Arrows and arrowheads in the parallel micrographs in panel E mark the position of cells whose cytoplasm or nuclei, respectively, is immunopositive for the antibody tested. Micrographs in all panels were taken with a 40x objective.