TABLE 2.
Distribution of 260 nm absorbance in Ribosomal Fractions of S. purpuratus eggs and early embryosa
Expt | Stage | Percentage inhibition with emetine |
Number of transits in emetine |
Percentage A260 nmb |
||
---|---|---|---|---|---|---|
Polyribosomes |
||||||
Monoribosomes | Small | Large | ||||
1. | UF eggs | Control (2) | 86.2 ± 1.7 | 8.4 ± 0.9 | 5.4 ± 0.8 | |
UFeggs | 90–70 (2) | 0.6/1.63 | 86.5 ± 0.8 | 7.5 ± 0.5 | 5.9 ± 0.3 | |
2. | Zygotes | Control | 72.0 | 12.4 | 15.6 | |
2-cell | Control | 68.2 | 13.6 | 18.2 | ||
2-cell | 75–40 | 2.3 | 69.9 | 16.8 | 13.3 | |
4-cell | Control | 69.5 | 13.9 | 16.6 | ||
4–8-cell | 75–40 | 5.0 | 70.7 | 13.5 | 15.9 | |
8–16-cell | Control | 69.3 | 13.9 | 16.8 | ||
16-cell | 75–40 | 6.4 | 69.8 | 13.5 | 16.7 | |
3. | Early Bl | Control (3) | 60.4 ± 3.5 | 25.5 ± 1.3 | 14.1 ± 2.3 | |
Early Bl | 72–40 | 1.3 | 44.5 | 30.5 | 24.7 |
Conditions and stages as in Table 1.
The distribution of material absorbing at 260 nm was determined graphically.