TABLE 3.
Expt | Stageb | Percentage inhibition with emetine |
Number of transits in emetine |
Percentage [3H]RNA |
||
---|---|---|---|---|---|---|
Polyribosomes |
||||||
Monoribosomes | Small | Large | ||||
1. | HatchingBlc | Control (2) | - | 42.5±0.3 | 26.4 ± 0.3 | 31.1 ± 0.6 |
Hatching Blc | 60 | 1.5 | 34.4 | 27.9 | 37.6 | |
2 | BlMy-1-2d | Control | - | 49.3 | 20.7 | 30.0 |
BlMy-1-2d | 72 | 1.05 | 31.4 | 22.8 | 45.8 | |
3. | BlMy-1e | Control (2) | - | 54.2 ± 0.1 | 18.9 ± 0.2 | 26.9 ± 0.2 |
BlMy-2/Ga-1f | Control | - | 58.2 ± 0.6 | 18.5 ± 0.5 | 23.3 ± 0.1 | |
BlMy-2f | 76 | 1.8 | 38.2 | 24.4 | 37.4 | |
4. | Prismg | Control (4) | - | 64.0 ± 1.7 | 20.6 ± 0.8 | 15.5 ± 1.5 |
Prismg | 77 (2) | 0.86/1.72 | 37.3 ± 1.0 | 27.5 ± 2.2 | 35.2 ± 1.2 |
Conditions as in Table 1.
For nomenclature of stages see Whiteley and Baltzer (1958). The culture times at 14°C at which polyribosome profiles were measured are:
16–18 hr,
23–24 hr,
24.5 hr,
27.5 hr,
48–52 hr.