TABLE 4.
Distribution of [3H]Rna in Ribosomal Fractions after ribonuclease and EDTA treatmenta
| Stage | Treatmentb | Percentage [3H]RNA |
||
|---|---|---|---|---|
| Polyribosomes |
||||
| Monoribosomes | Small | Large | ||
| UF eggs | None | 85.0 | 9.0 | 6.0 |
| +RNase | 86.9 | 7.7 | 5.4 | |
| +EDTA | 87.8 | 6.3 | 5.9 | |
| 2- to 4-cell | None | 63.3 | 15.5 | 21.2 |
| +RNase | 86.0 | 8.4 | 5.5 | |
| +EDTA | 87.2 | 7.6 | 5.2 | |
| Early Bl | None (3) | 58.9 ± 0.8 | 22.2 ± 1.0 | 18.8 ± 0.4 |
| +RNase (2) | 86.5 ± 0.1 | 8.0 ± 0.2 | 5.5 ± 0.1 | |
| +EDTA (2) | 90.7 ± 0.2 | 4.8 ± 0.1 | 4.5 ± 0.1 | |
a
Conditions as in Table 1.
b
Homogenates were treated with 29 µg/ml pancreatic ribonuclease A (Worthington) for 30–60 min at 0–4°C or made 35 mM EDTA with a 0.35 M stock just prior to loading on preformed gradients.