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. Author manuscript; available in PMC: 2015 Jul 10.
Published in final edited form as: Cell Rep. 2014 Jun 19;8(1):114–125. doi: 10.1016/j.celrep.2014.05.038

Figure 6. Functional IFN-response impacts VSV replication.

Figure 6

(A) 18–25 nucleotides (nts) RNA sequencing reads obtained from the lungs of mice infected intranasally with VSVctrl or VSV-VP55 (1×107 PFU for 24hrs) aligned for their distribution across the VSV-VP55 genome. “goi”, gene of interst. (B) Wildtype mice were infected intranasally with VSVctrl or VSV-VP55 (1×107 PFU; 3 mice per virus). 24hrs post infection lungs were analyzed for miR-146 and U6 expression by Northern blot. “M1, M2, M3”, mouse 1, mouse 2, mouse 3. Relative density depicts mature miR-146/U6. (C) Quantitative reverse transcriptase PCR depicting levels of IFN-β induction over endogenous tubulin levels from wildtype fibroblasts (MEF) infected with VSVctrl, VSV-VP55 or VSVNS1 (MOI of 1 for 16hrs). (D) Wildtype mice were infected intranasally with VSVctrl, VSV-VP55 or VSV-NS1 (1×107 PFU). 24hrs post infection lungs and spleens were analyzed by plaque assay. (E) Interferon I and III receptor knockout mice (Ifnar1−/−/Il28r−/−) were infected intranasally with VSVctrl, VSV-VP55 or VSV-NS1 (1×104 PFU). 48hrs post infection lungs and spleens were analyzed by plaque assay.