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. Author manuscript; available in PMC: 2015 Jul 10.
Published in final edited form as: Cell Rep. 2014 Jul 4;8(1):256–271. doi: 10.1016/j.celrep.2014.06.007

Figure 4. Mitochondrial activity is elevated in Rictor-deficient BAT.

Figure 4

(A) qRT-PCR of mitochondrial genes in P1 (n=6) and 6wk iBAT (n=8) (mean ± SEM; t-test; *p<0.05, **p<0.01)

(B) Differentially expressed genes using mitochondrial qRT-PCR arrays(n=4; t-test; p<0.05)

(C) Representative immunofluorescence images of Cox IV staining in 6wk iBAT (n=3).

(D) (Left) Representative TEM images of 6wk iBAT and (Right) mitochondria size (n=3;mean ± SEM; t-test; ***p<0.001)

(E) Oxygen consumption of iBAT using a Seahorse Flux Analyzer (12 wks, n=5; normalized to DNA content;mean ± SEM; t-test; *p<0.05)

(F) (Left) qRT-PCR of Ucp1 mRNA in iBAT with or without cold exposure (n=3 for 22°C; n=4 for 4°C; mean ± SEM; two-way ANOVA; ***P<0.001) and (Right) rectal temperature in acute cold challenge (n=4; mean ± SEM; t-test; no significant difference).

See also Figure S4.