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. Author manuscript; available in PMC: 2014 Jul 14.
Published in final edited form as: Oncogene. 2013 Jul 15;33(24):3172–3182. doi: 10.1038/onc.2013.279

Figure 1.

Figure 1

The activity of GSK3β inversely correlates with Slug protein levels. (a-b) EGF induces cell migration and invasion. A549 and HOP62 cells were cultured in serum-free medium for 12 h with or without EGF treatment for 6 h. Cell migration (a) and invasion (b) were determined by the Transwell assay at 6 h or 12 h after treatment. (c-d) Serum starvation reduces cell migration and invasion. A549 and HOP62 cells were cultured in DMEM or RPMI medium supplemented with 10% FBS or in serum-free medium for 6 h. Cell migration (c) and invasion (d) were determined by the Transwell assay at 6 h or 12 h after treatment. Data are shown as mean ± s.e.m. in three different experiments (n=3). (e-f) Correlation between the Slug protein level and the activity of GSK3β. Cell lysates of the experiments in (a-d) were analyzed by Western blotting.