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. 2014 May 1;197(3):839–849. doi: 10.1534/genetics.114.163279

Figure 3.

Figure 3

Rpb2-a is not associated with the Rpb1 complex at 18 hr postmixing. (A) Peptide spectrum match (PSM) scoring function for IgG compared to two replicates of Rpb2-IP at 18 hr. (B) Rpb1-IP at 12 and 18 hr postmixing, followed by Western blot analysis of Rpb2 and Rpb1, indicating the abundant presence of Rpb2 in the Pol-II complex at 12 and 18 hr. (C) Western hybridization of cell extracts from 12 hr postmixing, probed with Rpb1 or Rpb2 antibodies and exposed overnight to demonstrate the specificity of the antibody.